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Updated: Sep 25, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
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[Thyroglobulin (Tg)-specific induction of IL2 responsiveness in lymphocytes from patients with Hashimoto thyroiditis

I Yoshizawa, T Noma, K Maeda

    Arerugi = [Allergy]
    |November 1, 1989
    PubMed

    The peripheral blood mononuclear cells from Hashimoto thyroiditis patients acquired IL2 responsiveness in response to thyroglobulin stimulation, when they were partially depleted of adherent cells. Non-adherent cells reconstituted with Tg-pulsed autologous adherent cells at the ratio of 9:1 also acquired IL2 responsiveness, but those cultured with OVA (ovalbumin)-pulse adherent cells did not. This indicates Tg-induced IL2 responsiveness was specific for the antigen. Although there was no clear correlation between the intensity of induced IL2 responsiveness and the serum titer of anti-Tg antibody, Tg-induced IL2 responsiveness was significant in the seropositive patients. Pretreatment of the mononuclear cells with the monoclonal antibody to the HLA-DQ framework (Leu 10), but not the HLA-DR framework (OKIal), blocked the induction of IL2 responsiveness. This indicated that DQ-bearing adherent cells play a key role in presenting Tg antigen to the responder cells.

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