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CD100 and plexins B2 and B1 mediate monocyte-endothelial cell adhesion and might take part in atherogenesis

Maria Carolina A Luque1, Paulo S Gutierrez2, Victor Debbas2

  • 1Heart Institute of São Paulo (InCor), HC-FMUSP, São Paulo, SP, Brazil; Clinical Immunology and Allergy, Department of Clinical Medicine, University of São Paulo Medical School-HC-FMUSP, São Paulo, SP, Brazil.

Molecular Immunology
|August 16, 2015
PubMed

Insights

CD100 and plexins are key adhesion molecules in monocyte-endothelial cell binding, crucial for immune cell function and potentially atherosclerosis. Targeting these molecules may offer new therapeutic strategies.

Area of Science:

  • Immunology
  • Cell Biology
  • Cardiovascular Research

Background:

  • Leukocyte migration is vital for immune responses, involving interactions between leukocytes and endothelial cells (ECs).
  • Previous research identified adhesion molecules in this process, but CD100 and plexins' roles remain under investigation.
  • CD100 is implicated in platelet-endothelial cell interactions relevant to atherosclerosis.

Purpose of the Study:

  • To investigate the role of CD100 and plexins in monocyte-endothelial cell (EC) binding.
  • To identify potential receptors for CD100 on monocytes.
  • To explore CD100 and plexins as therapeutic targets in monocyte adhesion and atherosclerosis.

Main Methods:

  • Detected CD100 expression in monocytes, macrophages, foam cells, and endothelial cells.
  • Identified plexin B2 as a potential CD100 receptor on monocytes.
  • Utilized blocking experiments with antibodies against CD100, plexin B1, and plexin B2 in monocyte-EC adhesion assays.

Main Results:

  • CD100 is expressed on both monocytes and endothelial cells.
  • Plexin B2 was identified as a putative CD100 receptor on monocytes.
  • Blocking CD100, plexin B1, or plexin B2 significantly inhibited monocyte-EC adhesion.
  • CD100 and plexins function as adhesion molecules in monocyte-EC binding, potentially mediated by endothelial plexin B1 and monocytic plexin B2.

Conclusions:

  • CD100 and plexins play a significant role in monocyte-endothelial cell adhesion.
  • These molecules may be involved in the pathogenesis of atherosclerosis.
  • CD100 and plexins represent potential therapeutic targets for conditions involving monocyte adhesion.

Keywords:
AtherosclerosisMonocyte adhesionPlexinsSEMA4D

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