Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Do actin isoforms have unique functionalities at the protein level?

Nature communications·2025
Same author

A novel m.5906G > a variant in MT-CO1 causes MELAS/Leigh overlap syndrome.

Molecular genetics and genomics : MGG·2024
Same author

Heterogeneous Engineering Strategy Derived In Situ Carbon-Encased Nickel Selenides Enabling Superior LIBs/SIBs with High Thermal Safety.

ACS applied materials & interfaces·2024
Same author

Student Pharmacists' Perceptions of Artificial Intelligence and Machine Learning in Pharmacy Practice and Pharmacy Education.

American journal of pharmaceutical education·2024
Same author

Comprehensive pan-cancer analysis and experiments revealed R3HDM1 as a novel predictive biomarker for prognosis and immune therapy response.

Frontiers in genetics·2024
Same author

Selenium Improves Arsenic-Induced Male Reproductive Dysfunction by Regulating H3K14ac Level.

Biological trace element research·2024
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Apr 5, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

7.8K

High-Throughput Arginylation Assay in Microplate Format.

Sougata Saha1, Junling Wang, Anna S Kashina

  • 1Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Sonitpur, Assam, India.

Methods in Molecular Biology (Clifton, N.J.)
|August 20, 2015
PubMed
Summary
This summary is machine-generated.

A new biochemical assay enables high-throughput screening for arginyltransferase 1 (ATE1) inhibitors and activators. This method identified two compounds affecting ATE1-regulated processes in vitro and in vivo.

More Related Videos

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

12.4K
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

8.4K

Related Experiment Videos

Last Updated: Apr 5, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

7.8K
High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

12.4K
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

8.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Arginyltransferase 1 (ATE1) plays a crucial role in protein regulation.
  • Existing methods for studying ATE1 activity are limited in throughput and scope.

Purpose of the Study:

  • To develop a novel microplate-based biochemical assay for ATE1-mediated arginylation.
  • To enable high-throughput screening (HTS) for small-molecule modulators of ATE1.
  • To facilitate large-scale analysis of ATE1 substrates and related biological processes.

Main Methods:

  • Development of a microplate assay for in vitro ATE1-mediated arginylation.
  • Utilized beta-actin's N-terminal peptide as a model substrate.
  • Screened a library of 3280 diverse chemical compounds.

Main Results:

  • Successfully established a robust and scalable biochemical assay for ATE1.
  • Identified two distinct compounds that specifically modulate ATE1 activity.
  • Demonstrated the in vitro and in vivo efficacy of the identified compounds in affecting ATE1-regulated processes.

Conclusions:

  • The developed assay is suitable for high-throughput screening of ATE1 modulators.
  • The identified compounds represent potential leads for therapeutic interventions targeting ATE1 pathways.
  • This assay platform can be adapted for studying other ATE1 substrates and related enzymatic activities.