Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Preselection CD4+CD8+ thymocytes modulate TCR responsiveness following TCRβ selection.

Journal of immunology (Baltimore, Md. : 1950)·2026

Same author

Interleukin-17-Producing γδ T Cells Originate from SOX13<sup>+</sup> Progenitors that Are Independent of γδTCR Signaling.

Immunity·2026

Same author

Calibrating T cell responsiveness through interactions with self.

Nature reviews. Immunology·2026

Same author

Non-cognate CD8 Binding to MHC I Promotes Positive Selection of an MHC-E Restricted CD8 T Cell Population.

European journal of immunology·2026

Same author

Commensal-myeloid crosstalk in neonatal skin regulates interleukin-1 signaling and cutaneous type 17 inflammation.

Immunity·2026

Same author

Evidence for Maternal Autoantibodies in the Pathogenesis of Preterm Birth.

medRxiv : the preprint server for health sciences·2026

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 5, 2026

Preparation and Applications of Organotypic Thymic Slice Cultures

Preparation and Applications of Organotypic Thymic Slice Cultures

Published on: August 6, 2016

Studying T Cell Development in Thymic Slices.

Jenny O Ross¹, Heather J Melichar, Joanna Halkias

¹Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, 142 Life Sciences Addition, #3200, Berkeley, CA, 94720-3200, USA.

Methods in Molecular Biology (Clifton, N.J.)

|August 22, 2015

Summary

This summary is machine-generated.

Thymic slices offer a versatile method for studying T cell development in mouse and human systems. This technique enables high-throughput research on thymocyte differentiation in a well-established thymic environment.

More Related Videos

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Published on: June 9, 2015

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Published on: August 8, 2014

Related Experiment Videos

Last Updated: Apr 5, 2026

Preparation and Applications of Organotypic Thymic Slice Cultures

Preparation and Applications of Organotypic Thymic Slice Cultures

Published on: August 6, 2016

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Published on: June 9, 2015

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Published on: August 8, 2014

Area of Science:

Immunology
Developmental Biology
Cell Biology

Background:

Studying T cell development is crucial for understanding immune function.
Existing organotypic culture systems have limitations in studying thymocyte differentiation.
A need exists for accessible, high-throughput models of T cell development.

Purpose of the Study:

To describe methods for preparing and slicing thymic lobes.
To establish thymic slices as a versatile model for studying T cell development in situ.
To enable high-throughput analysis of thymocyte differentiation.

Main Methods:

Preparation of thymic lobes from mouse or human samples.
Embedding thymic tissue for cryosectioning.
Slicing embedded thymic lobes to create tissue slices.
Utilizing thymic slices for in situ studies of T cell development.

Main Results:

Thymic slices provide a technically accessible model for T cell development research.
The system supports high-throughput experiments.
Allows introduction of homogenous developmental intermediates into a structured thymic environment.
Facilitates in situ study of thymocyte differentiation.

Conclusions:

Thymic slices are a powerful and versatile tool for studying T cell development.
This method complements existing techniques for organotypic culture.
The thymic slice system is suitable for both mouse and human T cell development research.