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Sensitive quantitation of Ochratoxin A in cocoa beans using differential pulse voltammetry based aptasensor.
Rupesh K Mishra1, Akhtar Hayat2, Gaëlle Catanante1
1Laboratoire B.A.E, Université De Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
Food Chemistry
|August 26, 2015
Summary
A novel aptasensor detects Ochratoxin A (OTA) in cocoa beans using differential pulse voltammetry. This method offers sensitive and reliable OTA quantification, crucial for food safety.
Area of Science:
- Analytical Chemistry
- Biosensors
- Food Safety
Background:
- Ochratoxin A (OTA) is a mycotoxin found in cocoa beans, posing health risks.
- Accurate detection of OTA is essential for ensuring food safety and quality.
Purpose of the Study:
- To develop a sensitive and reliable aptasensor for Ochratoxin A detection in cocoa beans.
- To utilize differential pulse voltammetry (DPV) for signal transduction in the aptasensor.
Main Methods:
- A competitive aptasensor assay was designed using biotin-labeled OTA and immobilized aptamers on a screen-printed carbon electrode (SPCE).
- Avidin-alkaline phosphatase (ALP) conjugate and a substrate (1-naphthyl phosphate) were used for signal generation.
- Molecular imprinted polymer (MIP) columns were employed for sample extraction and purification.
Main Results:
- The aptasensor demonstrated good linearity from 0.15-5 ng/mL.
- A low limit of detection (LOD) of 0.07 ng/mL was achieved.
- High recovery rates (82.1-85%) with low relative standard deviation (RSD) of 3.87% were observed.
Conclusions:
- The developed aptasensor provides a sensitive and efficient method for Ochratoxin A detection in cocoa matrices.
- The combination of aptasensor technology and DPV offers a promising approach for food safety analysis.
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