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Imaging retinal densitometry with a confocal Scanning Laser Ophthalmoscope
D van Norren1, J van de Kraats
1Royal Netherlands Eye Hospital, Utrecht.
Vision Research
|January 1, 1989
Summary
This study introduces the Scanning Laser Ophthalmoscope (SLO) as a novel imaging retinal densitometer. The confocal SLO effectively measures retinal density differences, proving suitable for fundus reflectometry.
Area of Science:
- Ophthalmology
- Biomedical Optics
- Retinal Imaging
Background:
- The Scanning Laser Ophthalmoscope (SLO) is typically used for fundus imaging.
- Retinal densitometry is crucial for assessing visual pigment concentrations.
Purpose of the Study:
- To establish the Scanning Laser Ophthalmoscope (SLO) as an imaging retinal densitometer.
- To evaluate the capability of a confocal SLO for quantitative fundus reflectometry.
Main Methods:
- Utilized a confocal Scanning Laser Ophthalmoscope (SLO) with helium-neon or argon laser illumination.
- Raster scanned the laser beam across the retina, collecting descanned reflected light.
- Stored fundus images (22x18 degrees) in a computer, calculating density differences between dark-adapted and bleached states using 514 nm light.
Main Results:
- Quantified density differences in the fovea (approx. 0.35) and at 15-20 degrees eccentricity (approx. 0.15) after full bleaching.
- Observed significant density differences primarily in the foveal area following selective bleaching with 633 nm light.
Conclusions:
- The confocal Scanning Laser Ophthalmoscope (SLO) is a highly suitable instrument for imaging fundus reflectometry.
- This novel application of SLO enables quantitative assessment of retinal light absorption characteristics.