[Bacterial localization in apical cementum at the epithelial insertion using scanning electron microscopy].

Area of Science:

• Oral biology
• Microbiology
• Periodontology

Context:

• Chronic periodontitis leads to tooth loss in adults.
• Apical cementum integrity is crucial for tooth stability.
• Fractured teeth present diagnostic and treatment challenges.

Purpose:

• To investigate the microstructural changes in apical cementum of teeth extracted due to chronic periodontitis.
• To identify the presence and location of bacterial colonization within the apical cementum using scanning electron microscopy.

Summary:

• Adult teeth extracted for chronic periodontitis exhibited fractures.
• Scanning electron microscopy analysis of the apical cementum-junctional epithelium interface revealed bacterial presence.
• Bacteria were observed forming within microscopic niches in the apical cementum.

Impact:

• This study highlights a previously uncharacterized microstructural consequence of chronic periodontitis.
• Findings suggest that bacterial invasion of apical cementum may contribute to tooth fracture and extraction.
• Understanding these bacterial niches can inform future therapeutic strategies for periodontitis and tooth preservation.