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Flocculation in Saccharomyces cerevisiae is regulated by RNA/DNA helicase Sen1p

Vikash Singh1, Gajendra Kumar Azad1, Santhosh Kumar Sariki1

  • 1Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal 462023, India.

FEBS Letters
|September 15, 2015
PubMed

Insights

The Nrd1-Nab3-Sen1 (NNS) complex and Rrp6p regulate yeast flocculation genes. Cooperative roles of Rnt1p, Rrp6p, and the NNS complex repress FLO gene expression.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • RNA Processing

Background:

  • The Nrd1-Nab3-Sen1 (NNS) complex terminates non-coding RNA transcription and aids nuclear exosome degradation.
  • The NNS complex also represses stress response genes via premature termination.
  • Yeast flocculation involves cell aggregation mediated by lectin-encoding FLO genes.

Purpose of the Study:

  • To investigate the role of the NNS complex and Rrp6p in regulating flocculation (FLO) gene expression.
  • To explore the involvement of RNA processing machinery, specifically RNT1 and SEN1 mutants, in flocculation.

Main Methods:

  • Analysis of NNS complex and Rrp6p involvement in FLO1, FLO5, FLO9, and FLO10 gene expression.
  • Phenotypic analysis of deletion mutants for RNT1 and SEN1 mutants with impaired Rnt1p interaction.

Main Results:

  • The NNS complex and Rrp6p were demonstrated to play a role in the expression of FLO1, FLO5, FLO9, and FLO10.
  • Deletion mutants of RNT1 and SEN1 mutants unable to interact with Rnt1p exhibited a flocculation phenotype.
  • A cooperative role was identified for Rnt1p, Rrp6p, and the NNS complex in repressing FLO gene expression.

Conclusions:

  • Rnt1p, Rrp6p, and the NNS complex function cooperatively to repress FLO gene expression.
  • These factors are crucial regulators of the yeast flocculation process.
  • The study elucidates a novel regulatory network impacting stress response gene expression.
Keywords:
FlocculationGene regulationRNA helicaseSen1

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