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Immune monitoring technology primer: flow and mass cytometry

Holden T Maecker1, Alexandre Harari2

  • 1Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA USA.

Journal for Immunotherapy of Cancer
|September 18, 2015
PubMed
Abstract

No abstract available in PubMed .

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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