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Updated: Apr 3, 2026
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Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
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Culturing of Mouse Mesenchymal Stem Cells on Poly-3-Hydroxybutyrate Scaffolds
N V Andreeva1,2, A P Bonartsev3,4, I I Zharkova2
1V. A. Engelhard Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Bulletin of Experimental Biology and Medicine
|September 22, 2015
Abstract:
We studied the possibility of long-term culturing of mouse mesenchymal stem cells on a porous scaffold made of biocompatible polymer poly-3-hydroxybutyrate. The cells remained viable for at least 2 months and passed more than 65 population doublings in culture. Culturing on the scaffold did not change surface phenotype of cells. 3D poly-3-hydroxybutyrate scaffolds are appropriate substrate for long-term culturing of mesenchymal stem cells.
