A novel technique for staining bulk grids bearing ultrathin sections

Area of Science:

• Materials Science
• Microscopy Techniques

Background:

• Handling bulk grids for ultrathin section staining can be challenging.
• Current methods may lead to inefficiencies and safety concerns.

Purpose of the Study:

• To develop an improved technique for staining bulk grids.
• To enhance the efficiency, safety, and identification of grids during staining.

Main Methods:

• A silicone device comprising a base plate and a depression bar was designed.
• Bulk grids were systematically loaded into the base plate.
• The depression bar was used to create separated cells for staining and washing.

Main Results:

• The proposed technique facilitates orderly loading and separation of grids.
• Improved handling efficiency was observed during staining and washing.
• Enhanced safety and grid identification were achieved.

Conclusions:

• The silicone device offers a practical solution for bulk grid staining.
• This method optimizes the workflow for electron microscopy sample preparation.