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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Related Experiment Videos

Recent Progress in Fluorescent Imaging Probes.

Yen Leng Pak1, K M K Swamy2, Juyoung Yoon3

  • 1Department of Chemistry and Nano Sciences, Ewha Womans University, Seoul 120-750, Korea. xiaobai080488@yahoo.com.

Sensors (Basel, Switzerland)
|September 25, 2015
PubMed
Summary
This summary is machine-generated.

This review details advances in fluorescent imaging probes for detecting biologically vital molecules like biothiols and metal ions. These probes offer high selectivity and bioimaging capabilities for cell analysis.

Keywords:
fluorescent chemosensorsfluorescent chemosensors for ROSfluorescent chemosensors for biothiolsfluorescent chemosensors for metal ionsfluorescent probesimaging probes

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Area of Science:

  • Chemical Biology
  • Molecular Imaging
  • Analytical Chemistry

Background:

  • Fluorescence probes are valuable for detection due to simplicity, low limits, and cell bioimaging.
  • Molecular recognition and organic reactions have driven progress in fluorescent imaging probes.
  • Reaction-based fluorescent probes offer high selectivity for specific analytes.

Purpose of the Study:

  • To review recent progress in fluorescent imaging probes.
  • To highlight probes for biologically important species.
  • To showcase advancements in reaction-based fluorescent probe development.

Main Methods:

  • Utilizing molecular recognition principles.
  • Employing specific organic reactions for probe design.
  • Developing probes for bioimaging applications.

Main Results:

  • Demonstrated high selectivity of reaction-based probes.
  • Showcased probes for biothiols, reactive oxygen/nitrogen species.
  • Highlighted probes for metal ions (Zn2+, Hg2+, Cu2+, Au3+) and anions (cyanide, ATP).

Conclusions:

  • Fluorescent imaging probes are powerful tools for biological analysis.
  • Reaction-based probes provide enhanced selectivity and bioimaging capabilities.
  • Continued development promises further applications in understanding biological processes.