Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondria01:37

Mitochondria

21.4K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
21.4K
Mitochondria01:37

Mitochondria

4.3K
4.3K
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

5.0K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
5.0K
The Electron Transport Chain01:30

The Electron Transport Chain

21.5K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
21.5K
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

3.2K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
3.2K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

13.7K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lysosome-derived methylated arginine is a signalling metabolite controlling the lipidome.

Nature cell biology·2026
Same author

Metabolic control of smooth muscle cell phenotype switching in atherosclerosis.

bioRxiv : the preprint server for biology·2026
Same author

CES1 deficiency is associated with metabolic reprograming and endothelial dysfunction in pulmonary arterial hypertension.

American journal of respiratory and critical care medicine·2026
Same author

Systems genetics reveals ITIH5 as a key mediator of adipocyte-Endothelial crosstalk.

Molecular metabolism·2026
Same author

Sexual dimorphism shapes renal metabolic adaptation to a ketogenic diet.

Cell reports·2026
Same author

Intestinal interoceptive dysfunction drives age-associated cognitive decline.

Nature·2026
Same journal

Mitochondria produce lactate to vent redox pressure.

Trends in endocrinology and metabolism: TEM·2026
Same journal

Beyond fat storage: neuronal lipid droplets regulate whole-body metabolism.

Trends in endocrinology and metabolism: TEM·2026
Same journal

HDL resuscitates cells from ferroptosis.

Trends in endocrinology and metabolism: TEM·2026
Same journal

2-Methylbutyrylcarnitine (2MBC).

Trends in endocrinology and metabolism: TEM·2026
Same journal

Decoding growth hormone actions on human growth plate stem cells.

Trends in endocrinology and metabolism: TEM·2026
Same journal

Androgen loss backfires: Brain gate for tumor immunity.

Trends in endocrinology and metabolism: TEM·2026
See all related articles

Related Experiment Video

Updated: Apr 3, 2026

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe
08:07

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

Published on: May 2, 2025

1.1K

Mitochondria Cripple without Krüppel.

Cholsoon Jang1, Zolt Arany2

  • 1Perelman School of Medicine, University of Pennsylvania, 3400 Civic Blvd, Philadelphia PA 19104, USA; Harvard Medical School, 330 Brookline Ave, Boston MA 02215, USA.

Trends in Endocrinology and Metabolism: TEM
|September 26, 2015
PubMed
Summary
This summary is machine-generated.

Cardiac KLF4 is vital for heart health by maintaining mitochondria and clearing damaged ones. Disrupting KLF4 function leads to severe mitochondrial injury and heart failure, highlighting its critical role.

More Related Videos

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights
07:55

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Published on: June 16, 2023

2.3K
Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease
05:45

Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease

Published on: May 3, 2024

2.2K

Related Experiment Videos

Last Updated: Apr 3, 2026

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe
08:07

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

Published on: May 2, 2025

1.1K
Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights
07:55

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Published on: June 16, 2023

2.3K
Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease
05:45

Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease

Published on: May 3, 2024

2.2K

Area of Science:

  • Cardiovascular Biology
  • Mitochondrial Dynamics
  • Molecular Cardiology

Background:

  • Mitochondrial homeostasis is essential for cardiac function.
  • Dysfunctional mitochondria contribute to heart disease.
  • Mechanisms regulating mitochondrial quality control in the heart are not fully understood.

Purpose of the Study:

  • To investigate the role of cardiac KLF4 in mitochondrial biogenesis and clearance.
  • To elucidate the molecular pathways through which KLF4 influences mitochondrial homeostasis.
  • To determine the impact of KLF4 disruption on cardiac health and mitochondrial integrity.

Main Methods:

  • Utilized mouse models with cardiac-specific KLF4 disruption.
  • Investigated KLF4's interaction with the ERR/PGC-1 transcriptional coactivator module.
  • Assessed mitochondrial function, biogenesis, and mitophagy markers in cardiac tissue.
  • Evaluated cardiac structure and function following KLF4 perturbation.

Main Results:

  • Cardiac KLF4 plays a key role in maintaining mitochondrial homeostasis.
  • KLF4 interacts with the ERR/PGC-1 module to regulate mitochondrial biogenesis.
  • KLF4 is crucial for the clearance of damaged mitochondria through mitophagy.
  • Disruption of cardiac KLF4 results in profoundly injured mitochondria and leads to cardiac failure.

Conclusions:

  • Cardiac KLF4 is a critical regulator of mitochondrial quality control in the heart.
  • KLF4's interaction with ERR/PGC-1 and its role in mitophagy are essential for preventing mitochondrial injury and maintaining cardiac function.
  • Targeting KLF4 pathways may offer therapeutic strategies for heart disease associated with mitochondrial dysfunction.