[Simultaneous Determination of Three Components in Ficus microcarpa Leaves by HPLC]

To develop an HPLC method for the content determination of benzoic acid, 4-hydroxyphenylacetic acid and acetosyringonecas in Ficus microcarpa Leaves.

The determination was performed on Purospher® STAR C18 (250 mm x 4.6 mm,5 µm). The mobile phase was consisted of acetonitrile-0.2% phosphoric acid aqueous solution with linear gradient elution and the flow rate of 1.0 mL/min. The column temperature was set at 35 °C and the detection wavelength was 270 nm.

The linear range of benzoic acid, 4-hydroxyphenylacetic acid and acetosyringonecas was 0.0121-1.21 µg (r = 0.9995), 0.423-42.32 [Lg ( r = 0.9999) and 0.047-4.70 pg( r = 0. 9996) , respectively. The average recovery was 100.7, 101.2 and 96.5 respectively.

This method is simple,reproducible,and can be used for determination of three components of benzoic acid, 4-hydroxyphenylacetic acid and acetosyringonecas in Ficus microcarpa Leaves.