Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Controlling heterologous protein synthesis through a plant RNA ThermoSwitch.

Plant methods·2026
Same author

Unraveling the maturation pathway of a eukaryotic virus through cryo-EM.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Connecting In Vitro and In Vivo Studies of Biomolecular Interaction and Assembly.

Journal of molecular biology·2025
Same author

RNA elements and their biotechnological applications in plants.

The New phytologist·2025
Same author

The Specificity of RNA Packaging in Isometric RNA Plant Viruses is Principally Determined by Replication.

Journal of molecular biology·2025
Same author

How do RNA viruses select which RNA to package? The plant virus experience.

Virology·2025

Related Experiment Video

Updated: Apr 1, 2026

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

4.3K

A virus-based nanoplasmonic structure as a surface-enhanced Raman biosensor.

Nikolai Lebedev1, Igor Griva2, Walter J Dressick1

  • 1Center for Bio/Molecular Science and Engineering, US Naval Research Laboratory, 4555 Overlook Avenue SW, Code 6900, Washington DC 20375, USA; Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.

Biosensors & Bioelectronics
|October 4, 2015
PubMed
Summary

Researchers developed a virus-like particle (VLP) to precisely position gold nanoparticles in 3D, creating enhanced nanoplasmonic structures for sensitive biosensing applications.

Keywords:
Cowpea mosaic virusGold nanoparticlesRamanSERSSelf-assemblyVirus-like particles

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.6K
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

2.4K

Related Experiment Videos

Last Updated: Apr 1, 2026

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

4.3K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.6K
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

2.4K

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Materials Science

Background:

  • Localized surface plasmons on nanoscale structures enhance chem/bio sensor sensitivity.
  • Precise 3D positioning of metal nanoparticles in solution is challenging for complex nanoplasmonic structures.

Purpose of the Study:

  • To report a virus-like particle (VLP) for constructing 3D plasmonic nanostructures in solution.
  • To enable precise positioning of gold nanoparticles on VLPs using directed self-assembly.
  • To investigate the concentration of electromagnetic fields at "hot spots" and measure optical field spatial concentration efficiency.

Main Methods:

  • Directed self-assembly of gold nanoparticles onto virus-like particles (VLPs).
  • Fabrication of 3D plasmonic nanostructures in solution.
  • Measurement of optical field spatial concentration efficiency.
  • 3D finite element simulations.
  • DNA detection experiments.

Main Results:

  • Achieved precise positioning of gold nanoparticles on VLPs, forming 3D plasmonic nanostructures.
  • Demonstrated concentration of electromagnetic fields at inter-nanoparticle gaps ("hot spots").
  • Measured a ten-fold enhancement of capsid Raman peaks due to optical field concentration.
  • Experimental results aligned with 3D finite element simulations.
  • Successfully utilized nanoplasmonic structures for DNA detection down to 0.25 ng/microl.

Conclusions:

  • VLPs provide a platform for precise 3D assembly of nanoplasmonic structures in solution.
  • The fabricated structures exhibit efficient electromagnetic field concentration, enhancing Raman signals.
  • The nanoplasmonic structures show potential for sensitive DNA detection with internal protein-based tracing.