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Phosphoinositides and PIPs

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

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Related Experiment Video

Updated: Apr 1, 2026

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

The reason sickle reticulocytes expose PS.

Dennis E Discher¹, Paul A Ney²

¹UNIVERSITY OF PENNSYLVANIA;

|October 10, 2015

Summary

Phosphatidylserine (PS) exposure on sickle red blood cells is linked to reticulocyte maturation. This finding may help prevent blood clots in sickle cell disease (SCD).

Area of Science:

Hematology
Cell Biology
Disease Pathophysiology

Background:

Sickle cell disease (SCD) is a genetic blood disorder characterized by abnormal hemoglobin.
Thrombosis is a major complication in SCD, leading to significant morbidity and mortality.
Reticulocyte maturation is a critical process in red blood cell development.

Purpose of the Study:

To investigate the association between phosphatidylserine (PS) exposure and reticulocyte maturation in sickle erythrocytes.
To explore the potential of targeting PS exposure for preventing thrombosis in SCD.

Main Methods:

Analysis of phosphatidylserine (PS) exposure on sickle erythrocytes.
Correlation of PS exposure with specific markers of reticulocyte maturation.
Investigation of the role of PS in the context of sickle cell disease pathophysiology.

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Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

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Last Updated: Apr 1, 2026

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Published on: November 5, 2019

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

Main Results:

A direct link was established between phosphatidylserine (PS) exposure in sickle erythrocytes and a physiological event during reticulocyte maturation.
The study identified PS exposure as a key event in the maturation process of sickle red blood cells.

Conclusions:

The findings provide novel insights into the pathophysiology of sickle cell disease.
Targeting phosphatidylserine (PS) exposure represents a potential therapeutic strategy for preventing thrombosis in patients with sickle cell disease.