Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

12.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.5K
Next-generation Sequencing03:00

Next-generation Sequencing

101.1K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
101.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Normal life expectancy after all-trans retinoic acid and arsenic trioxide for acute promyelocytic leukemia.

Leukemia·2026
Same author

Exercise pulmonary hypertension in asymptomatic patients with HIV infection Insights from the RIGHT-NET registry.

European journal of internal medicine·2026
Same author

Mental health benefits of specific blue space types and characteristics: A systematic evidence map.

Environmental research·2026
Same author

Pharmacological and speech-language pathology management of dysphagia in patients with myasthenia gravis.

Naunyn-Schmiedeberg's archives of pharmacology·2026
Same author

Working hours and depression in the HEAF cohort.

Occupational medicine (Oxford, England)·2025
Same author

The sustainability of hypercholesterolemia treatment: New drugs have made such therapy more expensive.

Hipertension y riesgo vascular·2025
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
See all related articles

Related Experiment Video

Updated: Apr 1, 2026

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

11.1K

Deep sequencing in library selection projects: what insight does it bring?

J Glanville1, S D'Angelo2, T A Khan3

  • 1Program in Computational and Systems Immunology, Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford, CA, USA.

Current Opinion in Structural Biology
|October 10, 2015
PubMed
Summary
This summary is machine-generated.

High throughput sequencing revolutionizes antibody discovery and engineering. This technology enables deep insights into antibody libraries and selections, guiding improved antibody design and development.

More Related Videos

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

2.2K
Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
10:28

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Published on: July 15, 2018

10.2K

Related Experiment Videos

Last Updated: Apr 1, 2026

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

11.1K
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

2.2K
Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
10:28

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Published on: July 15, 2018

10.2K

Area of Science:

  • Biotechnology
  • Immunology
  • Bioinformatics

Background:

  • Antibody discovery and engineering are critical in therapeutics and diagnostics.
  • Traditional methods face limitations in scale and precision.
  • Advancements in sequencing technologies offer new possibilities.

Purpose of the Study:

  • To review the impact of high throughput sequencing on antibody discovery and engineering.
  • To outline key considerations for successful implementation of these technologies.
  • To discuss current challenges and future directions.

Main Methods:

  • High throughput sequencing for library design and selection analysis.
  • Polymerase Chain Reaction (PCR) reagent design.
  • Selection of appropriate sequencing platforms.
  • Computational tools and statistical analysis for data interpretation.

Main Results:

  • Enables creation of large, high-quality antibody libraries with billions of unique molecules.
  • Facilitates quantitative tracing of enriched clones during selection.
  • Provides position-specific insights into amino acid variations under selection pressure.
  • Allows for error removal, diversity estimation, and identification of selection signatures.

Conclusions:

  • High throughput sequencing significantly enhances antibody discovery and engineering processes.
  • Successful application requires careful attention to PCR reagents, sequencing platforms, and computational analysis.
  • Addressing remaining challenges will accelerate widespread adoption and further innovation.