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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Bio-sample detection on paper-based devices with inkjet printer-sprayed reagents.

Wun-Hong Liang1, Chien-Hung Chu1, Ruey-Jen Yang1

  • 1Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan.

Talanta
|October 14, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces a low-cost method for preparing paper-based analytical devices by spraying biochemical reagents using an inkjet printer. This automated approach simplifies large-scale production for diagnostics like aspartate aminotransferase (AST) and alanine aminotransferase (ALT) tests.

Keywords:
Bio-sample detectionColorimetryInkjet printerLow-costPaper-based analytical devicePoint-of-care

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Manual reagent introduction for paper-based analytical devices is time-consuming and inefficient for large-scale production.
  • Existing automated methods are often prohibitively expensive for widespread adoption.
  • There is a need for cost-effective, automated solutions for reagent deposition in paper-based diagnostics.

Purpose of the Study:

  • To develop and validate a low-cost method for automated reagent deposition on paper-based analytical devices.
  • To demonstrate the feasibility of using a modified inkjet printer for spraying biochemical reagents.
  • To assess the performance of inkjet-printed reagents for specific biochemical assays.

Main Methods:

  • A modified commercial inkjet printer was utilized to spray biochemical reagents directly onto 2D paper-based analytical devices.
  • Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) tests were performed using the prepared devices.
  • Colorimetric analysis coupled with image processing was employed to quantify the reaction products.
  • The influence of glucose concentration on detection performance was evaluated.

Main Results:

  • The inkjet spraying method successfully prepared paper-based analytical devices for AST and ALT detection.
  • Optimal observation times were determined: approximately 4 minutes for AST (0-105 U/l) and 1 minute for ALT (0-125 U/l).
  • The detection performance for both AST and ALT assays was found to be insensitive to varying glucose concentrations.

Conclusions:

  • Inkjet printing offers a low-cost, automated solution for reagent deposition on paper-based analytical devices.
  • This method enables efficient and reliable biochemical detection, suitable for large-scale diagnostic applications.
  • The developed technique provides a viable alternative to manual pipetting and expensive automated systems.