Aspartate Aminotransferase and Alanine Aminotransferase Detection on Paper-Based Analytical Devices with Inkjet...

Area of Science:

• Biochemistry
• Analytical Chemistry
• Materials Science

Background:

• Traditional pipette titration for paper-based microanalytical devices (PADs) is time-consuming for large-scale biochemical detection.
• Existing automated PAD production methods are often prohibitively expensive, limiting accessibility.
• There is a need for cost-effective and efficient methods for PAD manufacturing and biochemical analysis.

Purpose of the Study:

• To develop a low-cost method for manufacturing PADs using a modified inkjet printer for reagent application.
• To optimize reaction times for the detection of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) on these PADs.
• To validate the performance of the developed PADs for AST and ALT detection in human blood serum samples.

Main Methods:

• A modified inkjet printer was utilized to apply reagents onto paper-based microanalytical devices (PADs).
• Optimal reaction times for AST and ALT detection were determined through systematic experimentation.
• The PADs were tested for the quantitative detection of AST and ALT in real human blood serum samples.

Main Results:

• Optimal reaction times for AST and ALT detection were established as 6 and 7 minutes, respectively.
• The PADs demonstrated high linearity and accuracy for AST detection in the range of 5.4 to 91.2 U/L (R² = 0.9932).
• The PADs showed excellent linearity and accuracy for ALT detection in the range of 5.38 to 86.1 U/L (R² = 0.9944).
• Results from the proposed PADs for AST and ALT detection in serum samples showed good agreement with traditional spectrophotometric methods.

Conclusions:

• Inkjet printing offers a low-cost and efficient method for manufacturing PADs for biochemical analysis.
• The developed PADs provide a reliable and accurate platform for the detection of AST and ALT in human blood serum.
• This technology has the potential to improve the accessibility and efficiency of large-scale biochemical testing.