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Related Concept Videos

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
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Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
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Eltrombopag interference in routine chemistry testing.

Verena Gounden1, Zhen Zhao2

  • 1Department of Laboratory Medicine, National Institutes of Health Clinical Center, Bethesda, Maryland, USA verenagounden@yahoo.com.

Annals of Clinical Biochemistry
|October 23, 2015
PubMed
Summary
This summary is machine-generated.

High doses of Eltrombopag can interfere with routine chemistry tests, affecting results for cholesterol, triglycerides, and phosphate. This interference impacts clinical decisions, highlighting the need for awareness in laboratory diagnostics.

Keywords:
Eltrombopagchemistryinterference

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Area of Science:

  • Clinical Chemistry
  • Analytical Toxicology
  • Pharmacology

Background:

  • Eltrombopag, a thrombopoietin receptor agonist, is known to cause bilirubin interference at high doses.
  • A clinical laboratory investigated Eltrombopag's impact on routine chemistry assays after observing an unusual sample.

Purpose of the Study:

  • To evaluate the effect of Eltrombopag on a range of routine chemistry analytes.
  • To determine the extent of interference caused by Eltrombopag in spectrophotometric assays.

Main Methods:

  • Eltrombopag was spiked into serum samples at concentrations up to 500 µg/mL.
  • Multiple analytes, including lipids, phosphates, and liver enzymes, were measured using the Roche Cobas 6000 analyzer.
  • Interference was defined as a >10% change from baseline values.

Main Results:

  • Significant interference was observed for total cholesterol, triglycerides, inorganic phosphate, and high-density lipoprotein cholesterol.
  • Clinical significant interference was noted for total cholesterol, inorganic phosphate, and high-density lipoprotein cholesterol.
  • No interference was reported for other tested analytes like albumin, glucose, or electrolytes.

Conclusions:

  • High concentrations of Eltrombopag interfere with specific spectrophotometric assays on the Roche Cobas 6000.
  • Laboratory professionals should be aware of potential Eltrombopag interference when interpreting results for affected analytes.
  • Further investigation may be needed to confirm interference in other analytical systems.