Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

i-Dent: A virtual assistant to diagnose rare genetic dental diseases.

Computers in biology and medicine·2024

Same author

Characterization of Functional Materials Using Coherence Scanning Interferometry and Environmental Chambers.

ACS omega·2023

Same author

Functional phenotypes of CCR5 on CD4+ T cells of relevance to its genetic and epigenetic associations with HIV infection.

Cellular & molecular immunology·2020

Same author

Photonic jet lens.

Scientific reports·2019

Same author

Illumination conditions in microsphere-assisted microscopy.

Journal of microscopy·2019

Same author

Microsphere-assisted phase-shifting profilometry.

Applied optics·2017

Same journal

Three Noble Metal Nanoparticles Demonstrated Distinct Biological Interactions and Cell Death Pathways in Human Hepatocarcinoma Cell Lines.

Nanotechnology, science and applications·2026

Same journal

Parenteral Berberine vs Cisplatin- Loaded Lipid Nanoparticles - Development, Characterisation, Comparative Safety Profiling and Cytotoxicity in Cholangiocarcinoma Cell Models.

Nanotechnology, science and applications·2026

Same journal

Sacha Inchi Oil-Based Nanostructured Lipid Carriers for Curcumin Delivery: Development and Physicochemical Characterization.

Nanotechnology, science and applications·2026

Same journal

A Cost-Effective Approach to Triphenylamine-Assisted Fabrication of Stable Cu<sub>2</sub>O Nanoparticles: Structural Analysis and Multifunctional Antibacterial, Optical, and Electronic Performance.

Nanotechnology, science and applications·2026

Same journal

Surface-Modified Superparamagnetic Iron Oxide Nanoparticles (SPIONs) in a New Perspective for Prostate Cancer Therapy.

Nanotechnology, science and applications·2026

Same journal

Labeling of Amine-Modified Material Surfaces with PFB-Fluorescein.

Nanotechnology, science and applications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 31, 2026

Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Emerging optical nanoscopy techniques.

Paul C Montgomery¹, Audrey Leong-Hoi¹

¹Laboratoire des Sciences de l'Ingénieur, de l'Informatique et de l'Imagerie (ICube), Unistra-CNRS, Strasbourg, France.

Nanotechnology, Science and Applications

|October 23, 2015

Summary

This summary is machine-generated.

Optical nanoscopy offers advanced imaging solutions for healthcare. This study classifies techniques for superresolution and nanodetection, aiding biophysical and medical applications.

Keywords:

biophysics imaging medical imaging microscopy nanodetection superresolution

More Related Videos

Patterning via Optical Saturable Transitions - Fabrication and Characterization

Patterning via Optical Saturable Transitions - Fabrication and Characterization

Published on: December 11, 2014

Fabrication and Operation of a Nano-Optical Conveyor Belt

Fabrication and Operation of a Nano-Optical Conveyor Belt

Published on: August 26, 2015

Related Experiment Videos

Last Updated: Mar 31, 2026

Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Patterning via Optical Saturable Transitions - Fabrication and Characterization

Patterning via Optical Saturable Transitions - Fabrication and Characterization

Published on: December 11, 2014

Fabrication and Operation of a Nano-Optical Conveyor Belt

Fabrication and Operation of a Nano-Optical Conveyor Belt

Published on: August 26, 2015

Area of Science:

Optics
Biophysics
Medical Imaging

Background:

Modern healthcare demands advanced imaging with subcellular resolution or wide-field detection.
Far-field optical nanoscopy provides high-resolution and high-speed imaging solutions.
A growing number of optical nanoscopy techniques require a clear classification scheme.

Purpose of the Study:

To introduce a novel classification scheme for optical nanoscopy techniques.
To distinguish between superresolution and nanodetection methods.
To highlight emerging techniques and their applications in biophysics and medicine.

Main Methods:

Classification of optical nanoscopy into superresolution and nanodetection categories.
Review of techniques like digital holography and scattering lens microscopy for superresolution.
Categorization of nanodetection methods (contrast, phase, deconvolution, nanomarkers) with examples such as interference microscopy and stochastic fluorescence microscopy.

Main Results:

A classification scheme distinguishing superresolution and nanodetection is presented.
Superresolution is achieved in subcellular and in vivo imaging without labeling using techniques like digital holography.
Nanodetection methods, including phase and nanomarker-based approaches, reveal nanostructures and intracellular components.

Conclusions:

Optical nanoscopy techniques are crucial for addressing modern healthcare imaging challenges.
The proposed classification aids in understanding and applying diverse nanoscopy methods.
Emerging nanoscopy techniques offer significant potential for advancements in biophysics and medicine.