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RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

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MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

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miRNA Stability in Frozen Plasma Samples.

Francesca Balzano¹, Marta Deiana², Silvia Dei Giudici³

¹Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy. mariafrancesca22@virgilio.it.

Molecules (Basel, Switzerland)

|October 23, 2015

Summary

This summary is machine-generated.

MicroRNAs (miRNAs) are stable when frozen for extended periods. This study found that plasma miRNAs remained quantifiable after 14 years of frozen storage, indicating their long-term stability.

Keywords:

long freezing times miRNA sequences AU or UA stability

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression post-transcriptionally.
They play crucial roles in various biological processes and diseases.
Assessing miRNA stability in biological samples is vital for accurate research and diagnostics.

Purpose of the Study:

To evaluate the stability and quantity of specific plasma miRNAs after prolonged frozen storage.
To determine the long-term viability of miRNAs in samples stored for up to 14 years at -80 °C.

Main Methods:

Eight specific miRNAs were quantified in plasma samples from healthy controls.
Samples were analyzed before freezing and after storage periods of 6, 12 months, and 14 years at -80 °C.
A Life Technologies protocol was used for miRNA amplification and quantification from EDTA-treated blood.

Main Results:

The analyzed miRNAs demonstrated high stability over extended frozen storage periods.
Quantifiable amounts of miRNAs were still present in plasma samples frozen for 14 years.
The study indicates a long frozen half-life for the investigated plasma miRNAs.

Conclusions:

Plasma miRNAs are highly stable under long-term frozen storage conditions (-80 °C).
This stability supports the use of frozen plasma samples for miRNA research and biomarker discovery.
The findings validate the reliability of miRNA analysis in archived biological specimens.