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Updated: Mar 31, 2026

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
Published on: September 14, 2018
Expanding the scope of alkyne-mediated bioconjugations utilizing unnatural amino acids
Johnathan C Maza1, Zachary M Nimmo1, Douglas D Young1
1Department of Chemistry, College of William & Mary, Williamsburg, Virginia 23187, USA. dyoung01@wm.edu.
Abstract:
The importance of bioconjugates within the field of chemistry drives the need for novel methodologies for their preparation. Well-defined and stable bioconjugates are easily accessible via the utilization of unnatural amino acids (UAAs). As such, we have synthesized and incorporated two new UAAs into green fluorescent protein, and optimized a novel Cadiot-Chodkiewicz bioconjugation, effectively expanding the toolbox of chemical reactions that can be employed in the preparation of bioconjugates.
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