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Related Experiment Video

Updated: Mar 31, 2026

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

Oligonucleotide-directed mutagenesis for precision gene editing.

Noel J Sauer¹, Jerry Mozoruk¹, Ryan B Miller¹

¹Cibus, San Diego, CA, USA.

Plant Biotechnology Journal

|October 28, 2015

Summary

Oligonucleotide-directed mutagenesis (ODM) provides a rapid, precise, non-transgenic method for improving plant traits. This precision genome editing technology, part of the Rapid Trait Development System (RTDS), accelerates agricultural development to meet global food demands.

Keywords:

CRISPR RTDS ™TALEN oligonucleotide-directed mutagenesis precision gene editing

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Last Updated: Mar 31, 2026

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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Area of Science:

Agricultural Science
Molecular Biology
Genetics

Background:

Plant traits are influenced by genetic variations, including single nucleotide polymorphisms.
Enhancing agricultural productivity is crucial for global food security.
Traditional breeding methods can be slow; advanced techniques are needed to accelerate trait development.

Purpose of the Study:

To review the application of oligonucleotide-directed mutagenesis (ODM) as a precision genome editing technology.
To highlight ODM's role in trait improvement for agriculture.
To discuss the Rapid Trait Development System (RTDS) technology.

Main Methods:

Exploration of ODM's use in targeted DNA editing across various systems (bacterial, fungal, mammalian, plant).
Focus on ODM's application in plasmid, episomal, and chromosomal DNA.
Utilizing a fluorescence conversion system (BFP to GFP) to demonstrate precise single-nucleotide editing.

Main Results:

ODM enables precise, targeted edits in DNA using oligonucleotides.
A fluorescence conversion system demonstrated successful single-nucleotide editing (e.g., BFP to GFP).
In Arabidopsis thaliana protoplasts, ODM achieved up to 0.05% precisely edited GFP loci.

Conclusions:

ODM is a powerful, non-transgenic tool for agricultural trait improvement.
The Rapid Trait Development System (RTDS) leverages ODM for efficient genome editing.
Genome editing technologies like ODM are vital for enhancing crop performance and commercial plant varieties.