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[Determination of N-isopropylaniline in workplace air by high-performance liquid chromatography]
Objective:
To establish the method of high-performance liquid chromatography (HPLC) for the determination of N-isopropylaniline in workplace air.
Methods:
N-isopropylaniline in the air was collected by silicone tube, and was then dissolved by acetonitrile and determined by HPLC-UV detector.
Results:
There was a linear relationship within the range of 0.0-100.0 µg/ml with the method, and the regression equation was y=22 863x+10 665(r=0.999 9); the detection limit was 0.005 µg/ml, and the minimum detectable concentration was 1.7x10(-3) mg/m3 (3.0 L sampling volume); the average recoveries of standard addition were 96.2%-101.3%. The within-run precision was 2.31%-2.99%, and the between-run precision was 3.21%-4.55%. The average desorption efficiency was 97.6%, the breakthrough volume was more than 8.12 mg, the sampling efficiency waE 100%, and the samples could be stored for at least 7 days at room temperature.
Conclusion:
The indicators ol the method all meet the requirements of GBZ/T 210.4-2008 (Determination methods of air chemicals in workplace), and can be used for the determination of N-isopropylaniline in workplace air.
