Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spindle-pole organization during early mouse development.

L Hiraoka1, W Golden, T Magnuson

  • 1Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106.

Developmental Biology
|May 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Migraine and cardiovascular disease: a population-based study.

Neurology·2010
Same author

Laparoscopic antecolic Roux-en-Y gastric bypass with closure of internal defects leads to fewer internal hernias than the retrocolic approach.

Surgical endoscopy·2008
Same author

Characterization of mammary tumors from Brg1 heterozygous mice.

Oncogene·2007
Same author

Surgical intervention and understanding of diseases of the stomach and duodenum.

Current opinion in gastroenterology·2006
Same author

Breath biomarkers and non-alcoholic fatty liver disease: preliminary observations.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals·2006
Same author

A novel method for gene expression mapping of metastatic competence in human bladder cancer.

Neoplasia (New York, N.Y.)·2006
Same journal

The molecular mechanism underlying melatonin-mediated repair of ovarian damage in mice exposed to abnormal light cycles.

Developmental biology·2026
Same journal

The Drosophila ovarian terminal filament imports lipophilic molecules that support cyst and follicle development within the ovariole.

Developmental biology·2026
Same journal

Secreted Frizzled-Related Protein 1 Controls Distal Lung Formation via Wnt and PDGF Signaling.

Developmental biology·2026
Same journal

The vascular-osteogenic interface in craniofacial development: a structured review of emerging associations in congenital malformations.

Developmental biology·2026
Same journal

Turning off metamorphosis: Thyroid hormone deregulation in the evolution of obligately paedomorphic salamanders.

Developmental biology·2026
Same journal

Developmental analysis of the cone photoreceptor-less little skate retina reveals distinct Onecut1 isoforms.

Developmental biology·2026
See all related articles

Spindle pole organization changes during early mouse development. A mutation (oligosyndactyly) disrupts mitotic phosphoprotein staining in developing embryos.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Centrosomes are critical for spindle pole organization in early mammalian development.
  • Understanding centrosome dynamics is key to deciphering developmental processes and genetic mutations affecting them.

Purpose of the Study:

  • To investigate spindle pole organization during preimplantation mouse development.
  • To analyze the impact of the oligosyndactyly (Os) mutation on spindle pole components and mitotic phosphoproteins.

Main Methods:

  • Immunological detection of centrosomal components using specific antisera (centrin, NRS-01, 5051) and monoclonal antibodies (MPM-1, MPM-2).
  • Staining of unfertilized eggs, blastocysts, activated eggs, early cleavage-stage embryos, and oligosyndactyly mutant embryos.

Related Experiment Videos

  • Analysis of spindle pole and cytoplasmic staining patterns in mitotic cells.

    • Main Results:

    • Spindle poles showed differential staining with centrin, NRS-01, and 5051 across early developmental stages.
    • MPM-1 and MPM-2 intensely stained mitotic cell cytoplasm, with MPM-2 also staining spindle poles.
    • Oligondactyly (Os) homozygous mutant embryos exhibited altered MPM antibody staining in about half of mitotic cells, unlike wild-type embryos.

    Conclusions:

    • Spindle pole organization undergoes significant changes during early mouse embryogenesis.
    • The oligosyndactyly mutation affects mitotic phosphoprotein localization or stability, impacting cell division.
    • These findings highlight the dynamic nature of centrosomal components and their disruption in developmental mutants.