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Updated: Mar 31, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
A novel approach to enhance biological nutrient removal using a culture supernatant from Micrococcus luteus
Yindong Liu1, Xiaomei Su2, Lian Lu2
1Department of Environmental Engineering, Zhejiang University, Yuhangtang Road 866#, Hangzhou, 310058, People's Republic of China. liuyd2323@163.com.
Abstract:
A culture supernatant from Micrococcus luteus containing resuscitation-promoting factor (SRpf) was used to enhance the biological nutrient removal of potentially functional bacteria. The obtained results suggest that SRpf accelerated the start-up process and significantly enhanced the biological nutrient removal in sequencing batch reactor (SBR). PO4 (3-)-P removal efficiency increased by over 12 % and total nitrogen removal efficiency increased by over 8 % in treatment reactor acclimated by SRpf compared with those without SRpf addition. The Illumina high-throughput sequencing analysis showed that SRpf played an essential role in shifts in the composition and diversity of bacterial community. The phyla of Proteobacteria and Actinobacteria, which were closely related to biological nutrient removal, were greatly abundant after SRpf addition. This study demonstrates that SRpf acclimation or addition might hold great potential as an efficient and cost-effective alternative for wastewater treatment plants (WWTPs) to meet more stringent operation conditions and legislations.
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