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Updated: Mar 31, 2026
Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
Published on: April 20, 2017
A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein
Narjes Shokrollahi1, Delavar Shahbazzadeh1, Kamran Pooshang-Bagheri1
1Biotechnology Research Center, Venom and Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran.
Background:
Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells.
Methods:
The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells.
Results:
Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.
Conclusion:
The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.
