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Whole Mount Labeling of Cilia in the Main Olfactory System of Mice
Published on: December 27, 2014
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9 + 0 and 9 + 2 cilia are randomly dispersed in the mouse node
Toru Odate1, Sen Takeda2, Keishi Narita1
1Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimo-Kateau, Chuo, Yamanashi 409-3898, Japan.
Microscopy (Oxford, England)
|November 2, 2015
Summary
Most mouse embryonic node cilia are 9+0, with fewer 9+2 cilia randomly distributed. This study clarifies cilia structure and distribution, aiding understanding of left-right asymmetry determination.
Area of Science:
- Developmental Biology
- Cell Biology
- Embryology
Background:
- Left-right asymmetry is crucial for embryonic development.
- Cilia in the mouse node generate nodal flow, initiating left-right determination.
- Previous research suggested nodal cilia are 9+0, but 9+2 cilia have also been observed.
Purpose of the Study:
- To investigate the arrangement of 9+0 and 9+2 cilia within the mouse node.
- To determine the distribution patterns of these two cilia types.
- To explore the embryonic origin of node-surrounding crown cells.
Main Methods:
- Microscopic analysis of cilia structure in mouse embryos.
- Assessment of cilia distribution within the node.
- Investigation of crown cell origins.
Main Results:
- The majority of nodal cilia exhibit a 9+0 axonemal structure.
- Fewer 9+2 cilia were identified compared to 9+0 cilia.
- Both 9+0 and 9+2 cilia were found to be randomly distributed within the node.
Conclusions:
- Most nodal cilia are 9+0, with a minor population of 9+2 cilia.
- The random distribution of these cilia types suggests a non-polarized arrangement.
- Further research into crown cells may illuminate their role in node function.
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