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Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology
Published on: March 27, 2018
Detection of kidney damage by malathion impurities using a microdissection technique
S Keadtisuke1, W Dheranetra, T R Fukuto
1Department of Entomology, University of California, Riverside 92521.
Toxicology Letters
|April 1, 1989
Summary
Impurities in malathion, O,O,S-Trimethylphosphorothioate (OOS-Me) and O,S,S-trimethylphosphorodithioate (OSS-Me), cause delayed death in rats. Kidney examination revealed OOS-Me and OSS-Me induced significant kidney tubule damage.
Area of Science:
- Toxicology
- Nephrology
- Environmental Health
Background:
- O,O,S-Trimethylphosphorothioate (OOS-Me) and O,S,S-trimethylphosphorodithioate (OSS-Me) are known impurities in technical grade malathion.
- These impurities have been previously shown to induce delayed mortality in rats following oral administration.
Purpose of the Study:
- To investigate the mode of action of OOS-Me and OSS-Me.
- To examine the effects of these impurities on kidney structure at the nephron level.
Main Methods:
- Microdissection and microscopic examination of rat nephrons.
- Comparative analysis with kidney tissue from cadmium-chloride-treated rats.
Main Results:
- Nephrons from rats exposed to OOS-Me and OSS-Me exhibited significant swelling, distortion, and distension of glomeruli.
- A notable narrowing of the proximal tubule (swan neck region) was observed in treated rats.
- These pathological changes were comparable to those induced by cadmium chloride.
Conclusions:
- OOS-Me and OSS-Me are implicated in causing kidney tubule damage.
- The observed nephrotoxic effects suggest a potential mechanism for the delayed mortality associated with these malathion impurities.
