Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

recA-dependent DNA repair processes.

K C Smith, T C Wang

    Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
    |January 1, 1989
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Mild-moderate equine asthma: A scoping review of evidence supporting the consensus definition.

    Veterinary journal (London, England : 1997)·2022
    Same author

    Skin masses in dogs under one year of age.

    The Journal of small animal practice·2021
    Same author

    Cost-effectiveness of health coaching and financial incentives to promote physical activity after total knee replacement.

    Osteoarthritis and cartilage·2018
    Same author

    Combining online and in-person methods to evaluate the content validity of PROMIS fatigue short forms in rheumatoid arthritis.

    Quality of life research : an international journal of quality of life aspects of treatment, care and rehabilitation·2018
    Same author

    Cost-effectiveness of generic celecoxib in knee osteoarthritis for average-risk patients: a model-based evaluation.

    Osteoarthritis and cartilage·2018
    Same author

    An outbreak of norovirus GI-6 infection following a wedding in North West England.

    Epidemiology and infection·2017
    Same journal

    AI in Genomics: From Variant Calling to Multi-Omics Integration.

    BioEssays : news and reviews in molecular, cellular and developmental biology·2026
    Same journal

    Rethinking One Health: Microbial Foundations for Ecological Governance.

    BioEssays : news and reviews in molecular, cellular and developmental biology·2026
    Same journal

    Biobanked Liver Organoids: A Roadmap for Precision Hepatology.

    BioEssays : news and reviews in molecular, cellular and developmental biology·2026
    Same journal

    The Temporal Architecture of Human Cells: Organelle Clocks and Distributed Circadian Time.

    BioEssays : news and reviews in molecular, cellular and developmental biology·2026
    Same journal

    Opposing Activity at the Apical Surface: An Antagonistic Collaboration Between Crumbs and Myosin II Determines Organ Shape.

    BioEssays : news and reviews in molecular, cellular and developmental biology·2026
    Same journal

    Hidden Fungal DNA Structures May Shape Sequencing Outcomes.

    BioEssays : news and reviews in molecular, cellular and developmental biology·2026
    See all related articles

    The recA gene product in Escherichia coli repairs DNA damage from UV radiation, including excision gaps, daughter-strand gaps, and double-strand breaks. These repair mechanisms for different DNA lesions appear to be similar.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • UV radiation induces DNA lesions, including excision gaps, daughter-strand gaps, and double-strand breaks.
    • These lesions pose significant challenges to DNA replication and cellular integrity.
    • The recA gene product is a key player in DNA repair pathways.

    Purpose of the Study:

    • To investigate the role of the recA gene product in repairing various UV-radiation-induced DNA lesions in Escherichia coli.
    • To elucidate the mechanisms underlying recA-dependent repair of excision gaps, daughter-strand gaps, and double-strand breaks.
    • To determine if pre-replication repair of excision gaps involves similar mechanisms to post-replication repair.

    Main Methods:

    • Utilizing Escherichia coli as a model organism.

    Related Experiment Videos

  • Employing genetic analysis to study the recA gene and its product.
  • Investigating DNA repair processes through biochemical and molecular techniques.

    • Main Results:

    • The recA gene product is essential for the repair of all three types of UV-induced DNA lesions: excision gaps, daughter-strand gaps, and double-strand breaks.
    • Repair of daughter-strand gaps and double-strand breaks constitutes post-replication repair.
    • Evidence suggests that recA-dependent repair of excision gaps, occurring before replication, shares mechanisms with post-replication repair.

    Conclusions:

    • The recA gene product plays a central and versatile role in maintaining genomic stability following UV damage.
    • Escherichia coli employs conserved mechanisms for repairing different types of DNA lesions induced by UV radiation.
    • Understanding these repair pathways is crucial for comprehending cellular responses to genotoxic stress.