Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Deterministic DNA barcoding using vacuum-driven loading of free oligonucleotides to microwell arrays.

bioRxiv : the preprint server for biology·2025

Same author

EpiBlot: Joint Mapping of Chromatin Accessibility and Targeted Proteomics in HER2-expressing Breast Cancer Systems.

bioRxiv : the preprint server for biology·2025

Same author

Single-cell western blotting of cytoplasmic cytokeratin 8 proteoforms.

The Analyst·2025

Same author

Paired Analyses of Nuclear Protein Targets and Genomic DNA by Single-Cell Western Blot and Single-Cell PCR.

Analytical chemistry·2025

Same author

Paired Analyses of Nuclear Protein Targets and Genomic DNA by Single-Cell Western Blot and Single-Cell PCR.

bioRxiv : the preprint server for biology·2025

Same author

Single-cell western blotting of cytoplasmic cytokeratin 8 proteoforms.

bioRxiv : the preprint server for biology·2025

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 30, 2026

A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

Published on: August 22, 2018

Single-Cell Western Blotting.

Elly Sinkala¹, Amy E Herr²

¹Department of Bioengineering, University of California Berkeley, 306 Stanley Hall MC 1762, Berkeley, CA, 94720-1762, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 7, 2015

Summary

This summary is machine-generated.

Single-cell western blot (scWestern) offers a new method for protein analysis, overcoming limitations of antibody-based techniques. This assay enables highly specific, multiplexed protein detection in individual cells.

Keywords:

Immunoassay Immunoblot Lab-on-a-chip Proteomics Single cell

More Related Videos

Western Blotting: Sample Preparation to Detection

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Published on: September 26, 2020

Related Experiment Videos

Last Updated: Mar 30, 2026

A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

Published on: August 22, 2018

Western Blotting: Sample Preparation to Detection

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Published on: September 26, 2020

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Current single-cell protein analysis methods like flow cytometry and immunocytochemistry have limited specificity and multiplexing capabilities.
There is a need for advanced techniques to analyze protein expression at the single-cell level with high precision.

Purpose of the Study:

To introduce a novel single-cell western blot (scWestern) assay.
To overcome the limitations of existing protein analysis tools for single cells.

Main Methods:

Cells are settled into polyacrylamide microwells for lysis.
Polyacrylamide gel electrophoresis (PAGE) is performed through microwell walls for protein separation.
Photo-immobilization allows for antibody probing and archival storage of protein data.

Main Results:

The scWestern assay enables highly specific protein analysis by combining antibody detection with PAGE separation.
Simultaneous separation and analysis of over a thousand single cells are achievable.
Multiple protein targets can be investigated within individual cells.

Conclusions:

The scWestern assay provides a powerful new tool for single-cell protein analysis.
This method enhances specificity and multiplexing capabilities compared to traditional techniques.
The archival nature of scWestern allows for future protein target investigations.