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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Mar 30, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

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Aptamer Stainings for Super-resolution Microscopy.

Maria Angela Gomes de Castro¹, Burkhard Rammner¹, Felipe Opazo²

¹Department of Neuro- and Sensory Physiology, Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain, University of Göttingen Medical Center, Humboldtallee 23, 37073, Göttingen, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|November 11, 2015

Summary

This summary is machine-generated.

Aptamers offer superior accuracy for super-resolution microscopy compared to antibodies. This finding is crucial for visualizing cellular structures with advanced fluorescence microscopy techniques.

Keywords:

Affinity probes Antibodies Aptamers Microscopy STED Staining Super-resolution

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Last Updated: Mar 30, 2026

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Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

Area of Science:

Cell Biology
Microscopy
Biochemistry

Background:

Fluorescence microscopy allows visualization of molecules in biological contexts.
Conventional light microscopy is limited by light diffraction (~200 nm resolution).
Advanced techniques like stimulated emission depletion (STED) microscopy achieve ~10 nm resolution.

Purpose of the Study:

To compare the effectiveness of different affinity probes for live-cell super-resolution imaging.
To evaluate aptamers, antibodies, and transferrin for labeling transferrin receptors (TfnR).
To determine the optimal labeling strategy for high-resolution microscopy.

Main Methods:

Live staining of transferrin receptors (TfnR) using fluorescently labeled aptamers, monoclonal antibodies, and transferrin.
Imaging with conventional light microscopy (laser scanning confocal microscopy).
Imaging with super-resolution microscopy (stimulated emission depletion - STED microscopy).

Main Results:

Negligible differences in staining were observed between probes using conventional confocal microscopy.
Aptamers demonstrated superior performance over antibodies in STED super-resolution microscopy.
The smaller size of aptamers is advantageous for high-resolution imaging.

Conclusions:

Aptamers are superior to antibodies for super-resolution imaging due to their smaller size.
Advanced microscopy techniques like STED benefit from high-precision labeling strategies.
Aptamers represent a promising tool for detailed cellular imaging in biological research.