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Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo
Published on: September 11, 2018
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Human mesenchymal stem cell-engineered hepatic cell sheets accelerate liver regeneration in mice
Noriko Itaba1, Yoshiaki Matsumi1, Kaori Okinaka1
1Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.
Scientific Reports
|November 11, 2015
Summary
Hexachlorophene promotes liver cell development from mesenchymal stem cells (MSCs). Transplanted MSC-engineered hepatic cell sheets accelerate liver regeneration and improve survival in acute liver injury models.
Area of Science:
- Hepatology
- Stem Cell Biology
- Regenerative Medicine
Background:
- Mesenchymal stem cells (MSCs) show promise for cell therapy.
- The Wnt/β-catenin signaling pathway's role in hepatic differentiation requires further investigation.
Purpose of the Study:
- To investigate the potential of suppressing Wnt/β-catenin signaling to enhance hepatic differentiation of human MSCs.
- To develop and evaluate MSC-engineered hepatic cell sheets for treating acute liver injury.
Main Methods:
- Screening of 10 small molecule compounds using WST assay, TCF reporter assay, and albumin mRNA expression.
- Induction of hepatic differentiation in human MSCs using hexachlorophene.
- Orthotopic transplantation of MSC-engineered hepatic cell sheets in mouse models of acute liver injury.
Main Results:
- Hexachlorophene effectively suppressed Wnt/β-catenin signaling and induced hepatic differentiation of human MSCs.
- Transplantation of MSC-engineered hepatic cell sheets promoted liver regeneration and reduced injury in mice.
- Improved survival rates were observed in mice treated with cell sheets, with enhanced expression of complement C3, EGFR, and thioredoxin.
Conclusions:
- Hexachlorophene is a potent inducer of hepatic differentiation in human MSCs.
- MSC-engineered hepatic cell sheets represent a promising therapeutic strategy for acute liver injury.
- Liver regeneration is accelerated via complement C3, EGFR, and thioredoxin pathways post-transplantation.
