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Related Experiment Videos

Standardization of antimicrobial susceptibility testing.

A L Barry1

  • 1Clinical Microbiology Institute, Tualatin, Oregon.

Clinics in Laboratory Medicine
|June 1, 1989
PubMed
Summary

Early in vitro bacterial susceptibility testing methods lacked standardization, leading to inconsistent results. Coordinated efforts in the mid-20th century established standardized antimicrobial susceptibility testing in the US, improving clinical laboratory practices.

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Area of Science:

  • Clinical microbiology
  • Antimicrobial resistance
  • Laboratory science

Background:

  • In vitro methods for measuring bacterial susceptibility to antimicrobial agents were initially developed without coordinated efforts.
  • This led to a chaotic array of non-standardized and poorly controlled testing procedures across different laboratories.
  • The lack of standardization hindered reliable interpretation of antimicrobial susceptibility data.

Purpose of the Study:

  • To describe the historical development and standardization of antimicrobial susceptibility testing methods in the United States.
  • To highlight the challenges posed by early, uncoordinated testing approaches.
  • To acknowledge the efforts that led to a more sophisticated and standardized system.

Main Methods:

  • Historical review of the evolution of antimicrobial susceptibility testing methodologies.
  • Analysis of the impact of uncoordinated versus standardized approaches.
  • Examination of the role of key individuals and organizations in standardization efforts.

Main Results:

  • Early antimicrobial susceptibility testing was characterized by a lack of standardization and control.
  • Standardization efforts, particularly in the late 1950s and early 1960s, significantly improved testing reliability in the US.
  • The National Committee for Clinical Laboratory Standards now plays a crucial role in maintaining this standardization.

Conclusions:

  • The evolution of antimicrobial susceptibility testing demonstrates a progression from chaotic, individualized methods to a standardized, sophisticated system.
  • Standardization is essential for accurate and reproducible measurement of bacterial susceptibility to antimicrobial agents.
  • Ongoing efforts are necessary to maintain the high level of standardization in clinical laboratories.

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