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Srv2/CAP is required for polarized actin cable assembly and patch internalization during clathrin-mediated
Junko Y Toshima1, Chika Horikomi2, Asuka Okada2
1Department of Liberal Arts, Tokyo University of Technology, 5-23-22 Nishikamada, Ota-ku, Tokyo 144-8535, Japan Research Center for RNA Science, RIST, Tokyo University of Science, 6-3-1 Niijyuku, Katsushika-ku, Tokyo 125-8585, Japan.
Journal of Cell Science
|November 26, 2015
Summary
Srv2/CAP protein is crucial for yeast endocytosis, regulating actin patches and cables. Its domains ensure proper actin cytoskeleton organization for vesicle transport.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Actin filament dynamics are vital for vesicle trafficking in endocytosis.
- Yeast actin structures, cortical patches and cytoplasmic cables, are involved in endocytosis.
- Regulation of actin structure interaction in endocytosis is not fully understood.
Purpose of the Study:
- To investigate the role of Srv2/CAP, an actin regulator, in yeast endocytosis.
- To elucidate how Srv2/CAP influences the formation and interaction of actin patches and cables.
- To identify the specific domains of Srv2p responsible for its function and localization.
Main Methods:
- Gene deletion studies (SRV2 deletion).
- Microscopy to observe actin cytoskeleton organization (patches and cables).
- Analysis of protein domains (CARP domain, N-terminal helical domain) and their functions.
- Investigating protein interactions (Srv2p with cofilin).
Main Results:
- Srv2/CAP is essential for efficient endocytosis by regulating actin patch and cable formation.
- SRV2 deletion leads to fragmented actin cables that mislocalize relative to actin patches.
- The C-terminal CARP domain of Srv2p is critical for actin patch and cable assembly.
- The N-terminal domain of Srv2p mediates localization to ADP-actin regions via cofilin interaction.
Conclusions:
- Srv2p plays a key role in organizing the actin cytoskeleton for clathrin-mediated endocytosis in yeast.
- Specific domains of Srv2p are responsible for its distinct functions in actin structure regulation and localization.
- Srv2p acts as a molecular link between actin dynamics and vesicle transport during endocytosis.
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