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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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Co-translational assembly of protein complexes
Jonathan N Wells1, L Therese Bergendahl1, Joseph A Marsh2
1MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, U.K.
Biochemical Society Transactions
|November 29, 2015
Summary
Protein complex assembly can be coupled to translation on polysomes. This co-translational assembly occurs in prokaryotes and eukaryotes, impacting complex function and protein properties.
Area of Science:
- Cellular biology
- Molecular biology
- Biochemistry
Background:
- Biological macromolecules, especially proteins, interact to form stable complexes, crucial for cellular functions.
- The mechanisms of protein complex assembly within cells are not fully understood.
- Protein-protein interactions are fundamental to cellular life.
Purpose of the Study:
- To review evidence for protein complex assembly coupled to translation at the polysome.
- To discuss the implications of co-translational assembly for protein complex function.
- To identify protein properties associated with co-translational assembly.
Main Methods:
- Review of existing scientific literature on co-translational protein complex assembly.
- Analysis of studies demonstrating co-translational assembly in various organisms and cellular compartments.
- Identification of common characteristics of proteins undergoing co-translational assembly.
Main Results:
- Co-translational assembly of protein complexes occurs in both prokaryotic and eukaryotic organisms.
- This mechanism applies to both homomeric and heteromeric complexes.
- It is relevant for proteins translated into the cytoplasm and those targeted to or across membranes.
Conclusions:
- Co-translational assembly is a significant mechanism for protein complex formation.
- Understanding this process is key to comprehending cellular organization and function.
- Specific protein properties likely predispose them to co-translational assembly.
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