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Phospha-fluorescein: a red-emissive fluorescein analogue with high photobleaching resistance
Aiko Fukazawa1, Shinji Suda1, Masayasu Taki2
1Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. yamaguchi@chem.nagoya-u.ac.jp.
Abstract:
Phospha-fluorescein (POF), a phosphine oxide-containing analogue of fluorescein, was synthesized and its photophysical properties were examined. Compared with fluorescein and sila-fluorescein, POF displayed significantly red-shifted absorption and fluorescence as well as superior photobleaching resistance, while retaining the pH-responsive characteristics of fluorescein dyes.

