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Cyanogen bromide cleavage at methionine residues of polypeptides containing disulfide bonds
S Villa1, G De Fazio, U Canosi
1R-DNA Department, Instituto di Ricerca Cesare Serono, Ardea, Italy.
Analytical Biochemistry
|February 15, 1989
Abstract:
A method for cleaving polypeptides at their methionine residues without affecting intramolecular disulfide bonds is described. This method may be applied for cleaving recombinant heterologous hybrid polypeptides with release of the interesting peptide. The method may also be applied to assign the correct positions of disulfide bonds in protein molecules.