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Parallel, open-channel lateral flow (immuno) assay substrate based on capillary-channeled polymer films
Lynn X Zhang1, Liuwei Jiang, Daniel R Willett
1Department of Chemistry, Clemson University, Clemson, South Carolina 29634, USA. marcusr@clemson.edu.
The Analyst
|December 10, 2015
Summary
This study introduces novel capillary-channeled polymer (C-CP) films for bioaffinity separations. These films enable selective capture of target molecules in lateral flow assays, paving the way for multi-analyte testing.
Area of Science:
- Polymer Science
- Biotechnology
- Analytical Chemistry
Background:
- Lateral flow assays (LFAs) are widely used for rapid diagnostics.
- Existing LFA platforms often lack the capacity for multiplexed detection.
- Developing new materials for enhanced bioaffinity separations is crucial for advanced diagnostics.
Purpose of the Study:
- To present a novel implementation of functionalized polypropylene capillary-channeled polymer (C-CP) films.
- To establish C-CP films as a platform for lateral flow (immuno) assays.
- To demonstrate selective bioaffinity separations using modified C-CP films.
Main Methods:
- Fabrication of polypropylene C-CP films with parallel microchannels.
- Surface modification via UV treatment to enhance hydrophilicity.
- Physical adsorption of functionalized lipid tethered ligands (LTLs) as capture agents.
Main Results:
- Selective capture of Texas Red-labeled streptavidin (SAv-TR) using biotinylated-LTLs on UV-treated C-CP films.
- Demonstration of capture in <5 seconds with minimal non-specific binding of enhanced green fluorescence protein (EGFP).
- Confirmation of LTL-SAv conjugate formation using MALDI-MS.
Conclusions:
- C-CP films offer a promising platform for parallel, multi-analyte lateral flow assays.
- The described modification procedure is simple, performed under ambient conditions.
- This work lays the foundation for developing advanced multiplexed diagnostic devices.
