IMPACT OF GENISTEIN AND PHYTIC ACID ON THE VIABILITY AND PROLIFERATION ACTIVITY OF NASAL POLYPS' CELLS IN AN IN VITRO...

Area of Science:

• Biochemistry
• Cell Biology
• Otolaryngology

Background:

• Chronic rhinosinusitis with nasal polyps (CRSwNP) significantly impacts quality of life in developed countries.
• Current treatments for CRSwNP, including steroids and surgery, are often insufficient for persistent cases.
• Novel therapeutic agents that suppress inflammation and inhibit cell proliferation are needed for CRSwNP management.

Purpose of the Study:

• To investigate the in vitro effects of genistein and phytic acid on nasal polyp fibroblasts.
• To evaluate the impact of these compounds on fibroblast viability, growth rate, and apoptotic pathways.

Main Methods:

• Fibroblasts from nasal polyps were incubated with varying concentrations of genistein and phytic acid.
• Cell viability and growth were assessed using WST-1 and LDH assays after 72 hours.
• Gene expression (histone H3, BCL-2, BAX, P53) and caspase activity were analyzed via QRT-PCR and ELISA.

Main Results:

• Genistein and phytic acid significantly reduced nasal polyp fibroblast survivability and growth in a dose-dependent manner.
• Both agents decreased cell proliferation, as indicated by reduced histone H3 expression.
• Apoptosis was induced through modulation of BCL-2, BAX expression, and caspase-8 activity.

Conclusions:

• Genistein and phytic acid demonstrate significant potential as therapeutic agents for chronic rhinosinusitis.
• These compounds effectively inhibit the proliferation and viability of nasal polyp fibroblasts in vitro.
• The induction of apoptosis suggests a promising mechanism for future CRSwNP treatment strategies.