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Updated: Aug 3, 2026
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
[Determination of Amino Acids in Pearl Powder and Conch Powder from Different Origins by HPLC with Pre-column
Objective:
To establish an analysis method for determination of amino acids in pearl powder and conch powder from different origins by HPLC with pre-column derivation.
Methods:
Hydrolytic amino acids were extracted by acid hydrolysis method,then derivatized with phenyl isothiocyanate (PITC). And the samples were analyzed on a C, column with gradient elution of 0.1 mol/L sodium acetate solution (adjusted to pH 6. 5) (A) and acetonitrile (B) at the flow rate of 1. 0 mL/min. The detected wavelength was 254 nm.
Results:
Amino acids derivative solution remained stable in 24 hours. The response was linear for seven amino acids with a correlation coefficient r >0. 9995. The average recoveries were 93. 16% - 103. 31%.
Conclusion:
The method is sensitive with good accuracy and repeatability, which is useful for the determination of amino acids in pearl powder and conch powder from differei origins.
