Recoverable peroxidase-like Fe₃O₄@MoS₂-Ag nanozyme with enhanced antibacterial ability

Antibacterial agents with enzyme-like properties and bacteria-binding ability have provided an alternative method to efficiently disinfect drug-resistance microorganism. Herein, a Fe₃O₄@MoS₂-Ag nanozyme with defect-rich rough surface was constructed by a simple hydrothermal method and in-situ photodeposition of Ag nanoparticles. The nanozyme exhibited good antibacterial performance against E. coli (~69.4%) by the generated ROS and released Ag⁺, while the nanozyme could further achieve an excellent synergistic disinfection (~100%) by combining with the near-infrared photothermal property of Fe₃O₄@MoS₂-Ag. The antibacterial mechanism study showed that the antibacterial process was determined by the collaborative work of peroxidase-like activity, photothermal effect and leakage of Ag⁺. The defect-rich rough surface of MoS₂ layers facilitated the capture of bacteria, which enhanced the accurate and rapid attack of •OH and Ag⁺ to the membrane of E. coli with the assistance of local hyperthermia. This method showed broad-spectrum antibacterial performance against Gram-negative bacteria, Gram-positive bacteria, drug-resistant bacteria and fungal bacteria. Meanwhile, the magnetism of Fe₃O₄ was used to recycle the nanozyme. This work showed great potential of engineered nanozymes for efficient disinfection treatment.