[Recent development of Pichia pastoris system: current status and future perspective]

Area of Science:

• Biotechnology
• Molecular Biology
• Microbial Systems

Background:

• The Pichia pastoris yeast system has been developed over two decades.
• It is widely utilized in both laboratory and industrial settings.
• This system is crucial for various biotechnological applications.

Purpose of the Study:

• To review the advancements in the Pichia pastoris system.
• To cover progress in protein expression, molecular engineering, and biochemical production.
• To offer insights into current challenges and future prospects.

Main Methods:

• Literature review of Pichia pastoris development.
• Analysis of protein expression strategies.
• Examination of molecular engineering tools and methods.
• Assessment of biochemical production capabilities.

Main Results:

• Significant progress has been achieved in Pichia pastoris for protein expression.
• Advanced molecular engineering tools and methods have been developed.
• The system is effective for diverse biochemical production.

Conclusions:

• The Pichia pastoris system is a mature and versatile platform.
• Continued research is needed to address existing challenges.
• Future directions include further optimization for enhanced applications.