Comparison of the Fitting Validity Between the 2P Model and the Nondilute Solution Model Using Statistical Methods in...

Area of Science:

• Cellular Biology
• Biophysics
• Cryobiology

Background:

• Cell membrane transport is crucial for cryopreservation.
• Existing models like the two-parameter (2P) and nondilute solution models are used.
• A direct comparison of their fitting validity was lacking.

Purpose of the Study:

• To compare the fitting validity of the 2P model and the nondilute solution model.
• To determine the permeability parameters of porcine adipose-derived stem cells (pADSCs) using both models.
• To assess the predictive accuracy of each model against experimental data.

Main Methods:

• Determined pADSC permeability parameters using the 2P and nondilute solution models.
• Employed the Lilliefors test to evaluate the errors between model predictions and experimental data.
• Analyzed the mass transport across the cell membrane.

Main Results:

• The 2P model demonstrated slightly superior fitting validity compared to the nondilute solution model.
• The study identified differences in predictive accuracy between the two models.
• The Lilliefors test indicated the 2P model's better performance.

Conclusions:

• The 2P model is recommended for predicting mass transport across cell membranes in cryopreservation scenarios.
• The study provides insights into the strengths and weaknesses of each model.
• Further discussion on the causes of performance differences and model application is provided.