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Post-column reactors for sensitive and selective detection in high-performance liquid chromatography: categorization

U A Brinkman¹, R W Frei, H Lingeman

¹Department of Analytical Chemistry, Free University, Amsterdam, The Netherlands.

Journal of Chromatography

|August 11, 1989

Summary

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Automated on-line post-column reactors enhance rapid trace analysis in biomedical liquid chromatography. These systems improve selectivity, sensitivity, and reproducibility for complex sample analysis.

Area of Science:

Analytical Chemistry
Biomedical Analysis

Background:

Rapid trace analysis of biomedical samples is crucial.
Column liquid chromatography demands optimized sample pretreatment, separation, and detection.
Selectivity, sensitivity, and reproducibility are key analytical parameters.

Purpose of the Study:

To critically review on-line post-column reactors for liquid chromatography.
To categorize and evaluate different reactor types and their applications.
To compare on-line pre-column and post-column procedures in automated analytical systems.

Main Methods:

Review of theoretical background of on-line post-column reactors.
Categorization into open-tubular, packed-bed, segmented-stream, and hollow-fibre membrane reactors.

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Evaluation through selected applications and reaction types (electrochemical, photochemical, chemiluminescence, etc.).

Main Results:

On-line pre-column and post-column technologies significantly improve analytical performance.
Various reactor designs offer distinct advantages for specific applications.
Automation and specific reaction chemistries (e.g., derivatization, chemiluminescence) enhance detection capabilities.

Conclusions:

Automated on-line reactors are essential for efficient biomedical trace analysis.
The choice of reactor type and reaction strategy is critical for optimizing selectivity, sensitivity, and reproducibility.
On-line pre- and post-column procedures offer complementary benefits for advanced chromatographic analysis.