Related Experiment Video
Updated: Aug 2, 2026

10:47
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Published on: July 1, 2011
Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi
K E Hechemy1, H L Harris, J A Wethers
1Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Journal of Clinical Microbiology
|August 1, 1989
Abstract:
Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.

