Related Experiment Video
Updated: Mar 28, 2026

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
[Detection of complementary transcripts in intergenic region of the chicken α-globin gene domain]
E S Ioudinkova1,2, A V Nefedochkina3, O V Iarovaia1
1Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334;
Abstract:
Using strand-specific reverse transcription followed by Real Time PCR analysis we have characterized the transcription profile of the segment of chicken α-globin gene domain harboring embryonic gene π, adult gene αD and spacer region separating these genes. It has been demonstrated that in erythroid cells of adult lineage the spacer region is transcribed in both directions. These results suggest a possibility that switching of α-globin genes expression is controlled by RNA-interference mechanism.
Related Concept Videos
Complementation Tests
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
Complementary DNA
Complementary DNA
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...
Leaky Scanning

