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Patient-Specific Therapy via Cell-Reprogramming Technology: a Curative Potential for Patients with Diabetes
Haizhao Luo1, Xianbao Wang2, Ruyi Zhang3
1Department of Endocrinology, Nanhai Hospital, Southern Medical University, No. 40, Fo Ping Road, Foshan, 528200, China. luohaizhao@126.com.
Abstract:
Gene therapeutics provides great opportunities for curing diabetes. Numerous attempts have been made to establish a safe and high-efficiency gene delivery strategy, but all of them are unsuccessful. To achieve an ideal transfection, a novel gene delivery strategy was presented in this research. The novel system proposed was transfection mediated by the combination of ultrasound with microbubbles and cross-linked polyethylenimines (PEIs). Ultrasound with microbubbles enhances the permeability of target cells; moreover, cross-linked PEIs enabled DNA to escape from endosomes into the cytoplasm. If the proposed method is feasible and effective, the endogenous secretion system of insulin would be re-established in patients with diabetes.
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