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Aptamers-based sandwich assay for silver-enhanced fluorescence multiplex detection
Ying Wang1, Hui Li2, Danke Xu2
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, China; School of Environmental Science, Nanjing Xiaozhuang University, China.
Analytica Chimica Acta
|January 13, 2016
Summary
This study developed a highly sensitive protein detection method using aptamer-modified silver nanoparticles (AgNPs). This novel approach significantly enhances the detection limits for single and multiple proteins, offering improved diagnostic capabilities.
Area of Science:
- Biotechnology
- Nanotechnology
- Analytical Chemistry
Background:
- Developing sensitive and specific protein detection assays is crucial for diagnostics.
- Existing aptamer-based assays can be limited in sensitivity and require optimization.
- Silver nanoparticles (AgNPs) offer unique optical properties for signal enhancement.
Purpose of the Study:
- To develop a highly sensitive dual-aptamer sandwich assay for single and multiplex protein detection.
- To investigate the efficacy of aptamer-modified AgNPs as a capture substrate for protein detection.
- To compare the performance of AgNPs-based assays with traditional aptamer-based microarrays.
Main Methods:
- Preparation of aptamer-modified silver nanoparticles (AgNPs) as capture substrates.
- Synthesis of fluorescent dye-modified aptamers as detection probes.
- Development of a dual-aptamer sandwich assay for protein detection on a microarray platform.
Main Results:
- Aptamer-modified AgNPs significantly enhanced fluorescence detection sensitivity compared to aptamer-only microarrays.
- The sandwich assay achieved detection limits 80x and 8x lower for thrombin and platelet-derived growth factor-BB (PDGF-BB), respectively.
- Multiplex detection demonstrated limits of 625 pM for PDGF-BB and 21 pM for thrombin, indicating high sensitivity and specificity.
Conclusions:
- The dual-aptamer sandwich assay utilizing AgNPs is a highly sensitive and specific method for protein detection.
- This AgNPs-based approach offers superior performance for both single and multiplex protein analyses.
- The developed assay holds promise for advanced diagnostic applications requiring precise protein quantification.
